Table 3.
Summary of BMDExpress2 results for larval FHM high throughput exposure assays involving the full transcriptome and 10–12 doses per chemical with 8 replicates per dose. Transcriptomic point of departure (tPOD) calculated as the 10th percentile of the benchmark dose (BMD). Differentially expressed genes (DEGs), lower bound of the 95% confidence interval of the BMD (BMDL), upper bound of the 95% confidence interval of the BMD (BMDU), transcriptomics-based point of departure (tPOD).
| Chemical name | Input transcripts | DEGS pre-filter1 | DEGs post-filter2 | Median BMD (mg/L) | Median BMDL (mg/L) | Median BMDU (mg/L) | 10th Percentile tPOD (mg/L) |
|---|---|---|---|---|---|---|---|
| CuSO4 | 32,996 | 209 | 104 | 2.6e−2 | 1.8e−2 | 4.9e−2 | 5.1e−3 |
| NiSO4 | 31,449 | 158 | 33 | 2.2e−1 | 1.6e−1 | 4.6e−2 | 1.5e−1 |
| ZNso4 | 32,297 | 206 | 25 | 3.2e−4 | 1.1e−4 | 1.4e−3 | 6.3e−5 |
| Fluoxetine | 31,865 | 937 | 24 | 4.6e−4 | 1.1e−4 | 1.4e−3 | 3.2e−6 |
| Paroxetine | 31,884 | 498 | 313 | 7.2e−1 | 5.5e−1 | 1.0 | 4.4e−1 |
| Sertraline | 31,711 | 309 | 148 | 4.4e−1 | 3.1e−1 | 6.4e−1 | 1.2e−3 |
| Clothianidin | 31,158 | 13 | 3 | 3.8e−4 | 1.1e−4 | 2.6e−3 | 2.3e−4 |
| Flupyradifurone | 30,861 | 54 | 10 | 2.3e−5 | 2.3e−5 | 2.3e−5 | 2.3e−5 |
| Imidacloprid | 31,182 | 72 | 10 | 9.2e−2 | 2.9e−2 | 3.0e−1 | 3.5e−5 |
| Thiacloprid | 32,776 | 209 | 97 | 37 | 28 | 56 | 3.6e−2 |
Refers to number of genes significant by ANOVA and showing a trend via William’s trend test.
DEGs remaining after removing those with a global goodness of fit P-value ≤ 0.1; upper 95 % confidence bound of BMD/ lower 95 % confidence bound of BMD ≥ 40; BMD > highest concentration; and BMD < ten times lower than the lowest concentration tested.