Fig. 1.
Micropipette aspiration of GUVs. (A) Schematic representation of a micropipette capillary aspirating on a GUV. ΔP is the pressure difference between ambient pressure outside of the pipette and pressure inside the pipette. Dpip is the diameter of the pipette and Lp is the protrusion length of GUV at ΔP. (B) Representative brightfield image of an aspirated GUV to measure elastic area expansivity modulus. (C) Aspirated GUV with fluorescently labeled lipid bilayer (red) under iso-osmotic condition (left) and GUV immersed in a hyperosmotic buffer containing carboxyfluorescein (green) with a larger aspirated protrusion length (right). (D) Relationship between GUV and membrane curvature sensing protein (I-BAR) by modulating membrane tension using micropipette aspiration and optical tweezers for measure aspiration force and pulling membrane nanotubes, respectively. Panels B, C, D are adapted from [65], [70], and [71], respectively.