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. Author manuscript; available in PMC: 2023 Jan 6.
Published in final edited form as: Sci Immunol. 2022 Sep 23;7(75):eabl8357. doi: 10.1126/sciimmunol.abl8357

Fig. 7. Immune regulatory abnormalities in STK4-deficient patients.

Fig. 7.

(A) Schematic representation of STK4 illustrating its encoding exons, the protein domains, and mapped mutations of four patients. The kinase domain, the inhibitory domain and the SARAH (Sav/Rassf/Hpo) domain are indicated. (B and C) Flow cytometric analysis and cell frequencies of CD45RA and CCR7 expression on circulating Treg cells (B) and Teff cells (C) of control subjects (n=4) and STK4 deficient patients (n=5). (D) Cell frequencies of AnnexinV and Ki67 expression on circulating Treg and Teff cells of control subjects (n=4) and STK4 deficient patients (n=5 for AnnexinV and n=4 for Ki67). (E) Cell frequencies and MFI of CD69 and CD25 expression on circulating Treg cells of control and STK4 deficient patients (n=4/group). (F) Flow cytometric analysis and MFI of P65 expression on circulating Treg cells of control and STK4 deficient patients (n=4/group). (G) Representative histograms of MFI of phospho-S418 Foxp3 in circulating Treg cells of healthy control (HC) and STK4 deficient patients that were either unstimulated (US) or stimulated with anti-CD3 +IL-2 (n=4/group). Each dot represents one patient. Error bars indicate S.E.M. Statistical tests: Student’s two tailed t test (B to F) or one-way ANOVA with post-test analysis (G). *p<0.05, **,P<0.01, ***,P<0.001, ****,P<0.0001.