Skip to main content
. 2022 Dec 24;18:100527. doi: 10.1016/j.mtbio.2022.100527

Fig. 4.

Fig. 4

Characterization of sEVs obtained from different experimental groups. (A, B) TEM images of sEVs secreted by (A) untreated and (B) SEED-treated BM-MSCs (scale bars: 100 ​nm). (C) Western blot analysis on the protein markers CD81, CD9, TSG101, Calnexin, and GAPDH of sEVs from untreated (left column), SEED-treated (middle column) BM-MSCs, and cell lysate (right column). (D) Number of sEVs secreted by each untreated cell and each SEED-treated cell. (E) Quantification of protein amount in sEVs derived from each untreated cell and each SEED-treated cell. (F) Representative size distribution of sEVs isolated from BM-MSCs that are untreated (Control) and treated by the device (SEED), respectively. (G) Histogram on the size distribution of sEVs isolated from different experimental groups. (H) RT-qPCR analysis on expression levels of miRNAs involving in corneal epithelial wound healing. All error bars represent mean ​± ​standard error of the mean (N ​= ​3 devices, ∗P ​< ​0.05, ∗∗P ​< ​0.01, NS represents no statistical significance).