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Flow cytometry determination of the time course of the pore formation induced in human PMNs by increasing LukS-PV concentrations in the presence of LukF-PV. The pore formation is determined by the ethidium influx stimulated in 5 × 104 PMNs/ml by the addition of 0.01 to 100 nM LukS-PV and 30 nM LukF-PV.
