Figure 1: Inter-individual variation in metabolism.

a, Study outline. b, Volcano plots for subset of features detected by HPLC-MS (negative ion) in the exo-metabolomes for each wild strain versus the N2 reference strain, see Methods for more details. Arrows highlight iglu#93, a metabolite observed in the three wild strains but not in N2 (see also Extended Data Fig. 2). Dashed lines represent Benjamini-Hochberg adjusted significance thresholds at false discovery rates of 15% and 5%. c, Number of manually curated strain-specific metabolites, conservatively defined as any metabolite more than five-fold enriched or depleted in a given strain relative to the average of the other three strains at significance threshold p < 0.01 (unpaired, two-sided t-test). d, HPLC-MS (negative ion) extracted ion chromatograms (EICs) representing a homologous series of singly unsaturated hydroxy-fatty acids enriched in the wild strains (marked by arrows) relative to N2. A later-eluting isobaric series does not significantly vary between the four strains (marked by *, see also Extended Data Fig. 3). e, Quantification of 3HP in the endo- and exo-metabolome extracts of the four strains. Data represent six biologically independent experiments and bars indicate mean ± s.d., p-value calculated by unpaired, two-sided t-test with Welch correction. Source data are provided as a Source Data file.