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. 2022 Aug 18;22(2):213–228. doi: 10.1080/15384101.2022.2111929

Figure 4.

Figure 4.

The targeting relationship between lncRNA-UCA1 and miR-383, and miRNA effects on tumor cells proliferation and apoptosis. (a) Target relationship between miR-383 and lncRNA-UCA1 was predicted by bioinformatics analysis. (b) Luciferase reporter assay validated the target relationship between lncRNA-UCA1 and miR-383, miR-383 mimics significantly inhibit the fluorescence activity of reporter vector carrying the wild-type lncRNA-UCA1, but not the mutant. **P < 0.01 compared with NC group. (c) Gene expression level of miR-383 in cancer and normal tissues, miR-383 has significant low expression in tumor. **P < 0.01 comparison with adjacent group. (d) qRT-PCR analysis for UCA1 silenced HCC-78 cells, the expression level of miR-383 increased significantly after transfection. **P < 0.01 compared with NC group. (e) Validation result of miR-383 mimics increase its expression and miR-383 inhibitor reduce its expression. **P < 0.01 compared with Control group. (f) HCC-78 cells proliferation ability after transfected with different plasmids. Inhibiting miR-383 expression showed the highest in cells proliferations. (g–h) Colony formation assay presented a phenomenon that inhibiting miR-383 was likely to has low apoptosis rate and transfected cells with miR-383 mimics increased apoptosis. **P < 0.01 compared with Control group.