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Morphological differences between mammalian and avian spermatozoa (A - bull; B - rooster). Spermatozoa stained with PNA-lectin/FITC (acrosome, ACR), Hoechst-33342 (DNA), or Mitotracker Deep Red (mitochondria, MITO). A - frozen-thawed bull sperm. B - frozen-thawed rooster sperm. BF - bright field. Amnis ImageStream Imaging Flow Cytometer. Illustrative plots are shown.
