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. 2022 Dec 6;102(2):102386. doi: 10.1016/j.psj.2022.102386

Table 1.

Author 1 2 3 4 [%] 5 6 7 8 [%] 9 [%] 10 [%] 11 [%] 12 [%] 13 [%]
Abouelezz et al. (2017) Lake and Ravie S Gly 8 −5 to −100°C at 10°C/min, −196°C 3min/5°C 57.8 31.3
Lake and Ravie P DMA 5 −196°C 60°C 57 46.4
Abouelezz et al. (2015) Lake and Ravie S DMA 6 5 to −35°C at 7°C/min; −35°C to −140°C at 60°C/min 30sec/37°C 21.1 21.3 10.8
Lake and Ravie P DMA 6 −196°C 60°C 18 19.8 12.8
Lake and Ravie S Gly 11 5 to −35°C at 7°C/min; −35°C to −140°C at 60°C/min 3min/5°C 47.6 43.4 2.1
Lake and Ravie P Gly 11 −196°C 60°C 18.6 24 4.2
Lake and Ravie S Gly 8 5 to −35°C at 7°C/min; −35°C to −140°C at 60°C/min 3min/5°C 37 39 28.8
Lake and Ravie P DMA 3 −196°C 60°C 23.6 24.7 25
Appiah et al. (2020) modified P Gly 13.5 Quercetin −196°C 6sec/37°C
Appiah et al. (2019) modified P Gly 13.5 Melatonin −196°C 30sec/37°C
Behnamifar et al. (2021) Lake S DMA 4 5 to −35°C at 7°C/min; −35°C to −140°C at 60°C/min 3min30sec/5°C 15.3 14.08
Lake S Gly 8 5 to −35°C at 7°C/min; −35°C to −140°C at 60°C/min 3min30sec/5°C 40.22 52.17
Lake + BSA S Gly 8 5 to −35°C at 7°C/min; −35°C to −140°C at 60°C/min 3min30sec/5°C 35.4 25.06
Bernal et al. (2020) Lake and Ravie + Val S Gly 8 5 to −35°C at 7°C/min; −35°C to −140°C at 60°C/min 3 min/5°C 32.6
Blank et al. (2020) Lake + FBS S DMF 6 5°C for 15 min, −35°C for 12 min, −135°C for 5 min, −196°C 5 min/5°C 29.62
Ehling et al. (2012) HS1 S DMF + NMA 1:2 6,5/6,5 −3°C/min to −35°C, −50°C/min to −130°C at 60°C/min 4°C 44.3 81.1
HS1 S DMF + NMA 1:1 6,5/6,5 −3°C/min to −35°C, −50°C/min to −130°C at 60°C/min 4°C 47.7 77.3
HS1 S MA 6.5 −3°C/min to −35°C, −50°C/min to −130°C at 60°C/min 4°C 32.7 39.8
Fattah et al. (2017) BPSE S Gly 3 4 cm above the liquid N2 for 7 min 30 sec/37°C 52.7
BPSE S Gly 3 L carnitin 4 cm above the liquid N2 for 7 min 30 sec/37°C 69.1
Feyzi et al. (2018) Lake S Gly 5 5 cm above the liquid N2 for 12 min 30 sec/37°C 61.2 48
Lake + NO S Gly 5 5 cm above the liquid N2 for 12 min 30 sec/37°C 76.4 59
Gatti et al. (2021) Lake S DMA 3 3 cm above the liquid N2 for 7 min 20sec/37°C 37.2 58.4 70.3
Lake S DMA 6 3 cm above the liquid N2 for 7 min 20sec/37°C 42 53.7 74.6
Lake S DMA 9 3 cm above the liquid N2 for 7 min 20sec/37°C 34.8 55.1 74.4
Lake S DMA 12 3 cm above the liquid N2 for 7 min 20sec/37°C 32.4 50.7 65.8
Li et al. (2020) modified P Gly 13.5 Caragenan, CLC −196°C 37°C
Long et al. (2010) BPSE S Gly 11 −20°C for 30 min, −196°C 2-3min/3°C 49.6
Lotfi et al. (2017) BPSE S Gly 3 HA 4 cm above the liquid N2 for 7 min 46.1 55.3 65.5 57.5
Madeddu et al. (2016) Lake S DMA 6 1cm/3cm/5cm/7cm/10 cm above the liquid nitrogen for 10 min 30sec/38°C 46.1 31.1
Masoudi et al. (2021) Lake S Mito-tempo 5 cm above the liquid N2 for 12 min 30sec/37°C 65.1 62.3 65.3 87.3
Masoudi et al. (2019) Lake S Gly 3 Glu 4 cm above the liquid N2 for 7 min 30sec/37°C 60.8 58.8 63.8 49.8 91.4
Masoudi et al. (2018) Lake S Gly 3 Co-Q10 4 cm above the liquid N2 for 7 min 30sec/37°C 67.1 56.9 62.7 51.1 87.1
Mehaisen et al. (2020) EK P DMA 6 4°C for 15 min (1.13°C/min), −196°C 6sec/60°C 32.5 34.7 64.6
Mehdipour et al. (2021) Lake+AFP3 S Gly 3.8 4 cm above the liquid N2 for 7 min 30sec/37°C 61.5
Mehdipour et al. (2018) BPSE S SBL 0.5 4 cm above the liquid N2 for 7 min 30sec/37°C 43.04 41.79 41.39
BPSE S SBL 1 4 cm above the liquid N2 for 7 min 30sec/37°C 73.56 68.74 54 61.65
BPSE S SBL 1.5 4 cm above the liquid N2 for 7 min 30sec/37°C 55.7 50.61 46.85
BPSE S EY 10 4 cm above the liquid N2 for 7 min 30sec/37°C 56.76 53.76 47.71
BPSE S EY 15 4 cm above the liquid N2 for 7 min 30sec/37°C 64.96 59.5 55.49
BPSE S EY 20 4 cm above the liquid N2 for 7 min 30sec/37°C 76.03 70.12 60 66.26
Mehdipour et al. (2020) Lake S Gly 2 P188 4 cm above the liquid N2 for 7 min 30sec/37°C 66.7
Lake S Gly 8 P188 4 cm above the liquid N2 for 7 min 59
Mphaphathi et al. (2016) Kobidil+ S DMSO 8 5 to −20°C at −1°C/min; 5 cm above the liquid N2 for 5 min, −196°C 5min/5°C 46
Kobidil+ S EG 8 5 to −20°C at −1°C/min; 5 cm above the liquid N2 for 5 min, −196°C 5min/5°C 45
Kobidil+ S PND 8 5 to −20°C at −1°C/min; 5 cm above the liquid N2 for 5 min, −196°C 5min/5°C 21.8
Mosca et al. (2016) Lake S DMA 6 3 cm above the liquid N2 for 10 min 30sec/38°C 46.9 35.8
Lake+T 0,1M S DMA 6 3 cm above the liquid N2 for 10 min 30sec/38°C 46.4 38.4
Lake+S 0,1M S DMA 6 3 cm above the liquid N2 for 10 min 30sec/38°C 44.7 35.3
Lake +S/T (0,1M; 0,1M) S DMA 6 3 cm above the liquid N2 for 10 min 30sec/38°C 43.4 35.9
Mosca et al. (2020) Lake+T 0,1M S NMA 6 3 cm above the liquid N2 for 10 min 100sec/5°C 50.7 52.3
Lake+T 0,1M S NMA 9 3 cm above the liquid N2 for 10 min 100sec/5°C 36.6 35.5
Lake+T 0,1M S NMA 6 3 cm above the liquid N2 for 10 min 30sec/38°C 22.8 20
Lake+T 0,1M S NMA 9 3 cm above the liquid N2 for 10 min 30sec/38°C 20.5 18.1
Miranda et al. (2018) Kobidil+ S DMA 6 5 cm above the liquid N2 for 15 min 30sec/37°C 32.3
Kobidil+ S DMF 7.5 5 cm above the liquid N2 for 15 min 30sec/37°C 28.6
Kobidil+ S MA 9 5 cm above the liquid N2 for 15 min 30sec/37°C 30.7
Kobidil+ S EG 8 5 cm above the liquid N2 for 15 min 30sec/37°C 27.9
Kobidil+ S DMA 6 5 cm above the liquid N2 for 15 min 2min/5°C 31.1
Kobidil+ S DMF 7.5 5 cm above the liquid N2 for 15 min 2min/5°C 39.8
Kobidil+ S MA 9 5 cm above the liquid N2 for 15 min 2min/5°C 31.3
Kobidil+ S EG 8 5 cm above the liquid N2 for 15 min 2min/5°C 46.6
Nabi et al. (2016) Nabi S Gly 3 4 cm above the liquid N2 for 7 min 3min/4°C 65.4 73.1 53.5
BPSE S Gly 3 4 cm above the liquid N2 for 7 min 3min/4°C 21.4 21
Najafi et al. (2021) Lake+SBL S DMSO 4 P188 4 cm above the liquid N2 for 7 min 30sec/37°C 79.5
Najafi et al. (2019) BPSE S Gly 3.8 Resveratrol 4 cm above the liquid N2 for 7 min 30sec/37°C 70.8 64.3 58.1 64.5
BPSE S Gly 3.8 Resveratrol+NLC 4 cm above the liquid N2 for 7 min 30sec/37°C 75 73 67.2 67.3
Najafi et al. (2018) BPSE S Gly 8 Lycopene 4 cm above the liquid N2 for 7 min 30sec/37°C 69.7 64.08 57.8 59.22
BPSE S Gly 8 Lycopene LnL 4 cm above the liquid N2 for 7 min 30sec/37°C 72.91 68.07 62.2 64.66
Partyka et al. (2017) EK P DMA 6 L-carnitin 1mM −8°C for 15 min, −196°C 6sec/60 °c 37.6 48.06
EK P DMA 6 L-carnitin 5mM −8°C for 15 min, −196°C 6sec/60 °c 34.33 49.94
EK P DMA 6 Hypotaurin 1mM −8°C for 15 min, −196°C 6sec/60 °c 36.44 52.18
EK P DMA 6 Hypotaurin 10mM −8°C for 15 min, −196°C 6sec/60 °c 32.83 51.4
EK P DMA 6 Taurin 1mM −8°C for 15 min, −196°C 6sec/60 °c 40.18 53.58
EK P DMA 6 Taurin 10mM −8°C for 15 min, −196°C 6sec/60 °c 35.41 53.37
Rakha et al. (2018) RFE S EY 10 37°C to 4°C (−0.275°C/min), 4°C to −80°C (−8.4°C), −196°C 30sec/37°C 61.2 75.9 75.6
RFE S EY 15 37°C to 4°C (−0.275°C/min), 4°C to −80°C (−8.4°C), −196°C 30sec/37°C 74.1 75 55.9 75.9
RFE S EY 20 37°C to 4°C (−0.275°C/min), 4°C to −80°C (−8.4°C), −196°C 30sec/37°C 67.1 63.5 70.4
RFE S EY 25 37°C to 4°C (−0.275°C/min), 4°C to −80°C (−8.4°C), −196°C 30sec/37°C 74.8 65.1 62
RFE S Gly 20 37°C to 4°C (−0.275°C/min), 4°C to −80°C (−8.4°C), −196°C 30sec/37°C 61.9 51.8 46.5 65.6
Rakha et al. (2018) modifided S Gly 20 37°C to 4°C (−0.275°C/min), 4°C to −80°C (−8.4°C), −196°C 30sec/37°C 53.1
modifided S DMSO 4 37°C to 4°C (−0.275°C/min), 4°C to −80°C (−8.4°C), −196°C 30sec/37°C
modifided S DMSO 6 37°C to 4°C (−0.275°C/min), 4°C to −80°C (−8.4°C), −196°C 30sec/37°C
modifided S DMSO 8 37°C to 4°C (−0.275°C/min), 4°C to −80°C (−8.4°C), −196°C 30sec/37°C 73
modifided S DMSO 10 37°C to 4°C (−0.275°C/min), 4°C to −80°C (−8.4°C), −196°C 30sec/37°C
Salehi et al. (2020) Lake S Gly 3 4 cm above the liquid N2 for 5 min 30sec/37°C 60 77.2 59.3 32.9
BPSE S Gly 3 4 cm above the liquid N2 for 5 min 30sec/37°C 51 68.3 47.2 35.5
Murugesan and Mahapatra (2020) Sasaki S DMA 6 4.5 cm above the liquid N2 for 30 min 100sec/5°C 34.93 0 4.57
Sasaki S DMSO 2 4.5 cm above the liquid N2 for 30 min 100sec/5°C 22.66 0 94.85
Lake S DMA 6 4.5 cm above the liquid N2 for 30 min 100sec/5°C 34.46 0 4.14
Lake S DMSO 2 4.5 cm above the liquid N2 for 30 min 100sec/5°C 21.23 0 91.61
Sasaki S EG 8 4.5 cm above the liquid N2 for 30 min 100sec/5°C 23.72 0 94
Lake and Ravie S EG 8 4.5 cm above the liquid N2 for 30 min 100sec/5°C 26.52 0 92.33
RFE S EG 8 4.5 cm above the liquid N2 for 30 min 100sec/5°C 22.72 0.83 92.83
Sasaki S EG 8 4.5 cm above the liquid N2 for 30 min 30sec/37°C 26 18.39 84.58
Lake and Ravie S EG 8 4.5 cm above the liquid N2 for 30 min 30sec/37°C 24.67 48.12 69.98
RFE S EG 8 4.5 cm above the liquid N2 for 30 min 30sec/37°C 20.33 38.29 62.73
Sasaki S DMF 6 4.5 cm above the liquid N2 for 30 min 100sec/5°C 26 24.76 55.61
Lake and Ravie S DMF 6 4.5 cm above the liquid N2 for 30 min 100sec/5°C 23.81 30.89 22.03
BPSE S DMF 6 4.5 cm above the liquid N2 for 30 min 100sec/5°C 23.53 19.32 12.8
Shanmugam and Mahapatra (2019) Lake and Ravie P DMF 6 −196°C 45sec/56°C 28.31 1.19
Lake and Ravie P DMF 9 −196°C 45sec/56°C 24.02 1.38
TES/NaCl P DMF 6 −196°C 45sec/56°C 17.79 0
TES/NaCl P DMF 9 −196°C 45sec/56°C 19.51 0
Lake and Ravie P DMA 6 −196°C 60°C 40.23 2.75
Lake and Ravie P DMA 9 −196°C 60°C 39.62 9.22
Lake and Ravie+S+BSA P DMA 6 −196°C 60°C 39.62 9.58
Lake and Ravie+S+BSA P DMA 9 −196°C 60°C 41.81 7.6
modifide P DMA 6 −196°C 60°C 38.71 9.81
modifide P DMA 9 −196°C 60°C 36.64 3.1
RFE S NMA 12 4.5 cm above the liquid N2 for 30 min 100sec/5°C 24.43 0
RFE S DMA 9 4.5 cm above the liquid N2 for 30 min 100sec/5°C 27.85 0
RFE S DMSO 4 4.5 cm above the liquid N2 for 30 min 100sec/5°C 27.21 1.9
Shahverdi et al. (2015) BPSE S Gly 2 5 cm above the liquid N2 for 12 min 30sec/37°C 22.7
BPSE S LDL 1 5 cm above the liquid N2 for 12 min 30sec/37°C 24.1
BPSE S LDL 2 5 cm above the liquid N2 for 12 min 30sec/37°C 32.2
BPSE S LDL 4 5 cm above the liquid N2 for 12 min 30sec/37°C 43.1
BPSE S LDL 6 5 cm above the liquid N2 for 12 min 30sec/37°C 32.1
BPSE S LDL 8 5 cm above the liquid N2 for 12 min 30sec/37°C 19.8
Stanishevskaya et al. (2021) LCM P DMA 6 −196°C 60°C 48 79
LCM+4,8mM P DMA 6 −196°C 60°C 48 82
LCM+9,5mM P DMA 6 −196°C 60°C 50 86
Thélie et al. (2019) Lake S Gly 11 −7°C/min 3min/4°C 83.3
BHSV S EG 10 −1°C/min 3min/4°C 1.5
BHSV S DMF 6 −15°C/min 3min/4°C 64.8
FEB S DMA 6 −60°C/min 5sec/40°C 35.3
Wu et al. (2020) Lake S Gly 2 −5°C/min, −9°C/min, −13°C/min, −6.5°C/min 3min/5°C 34.8
Lake S Gly 4 −5°C/min, −9°C/min, −13°C/min, −6.5°C/min 3min/5°C 75.1
Lake S Gly 6 −5°C/min, −9°C/min, −13°C/min, −6.5°C/min 3min/5°C 77.63
Lake S Gly 8 −5°C/min, −9°C/min, −13°C/min, −6.5°C/min 3min/5°C 70.09
Lake S Gly 11 −5°C/min, −9°C/min, −13°C/min, −6.5°C/min 3min/5°C 57.43

1) extender (BSA- bovine serum albumine; Val-valine; FBS-fetal bovine serum; NO-nitric oxide; BPSE-Beltsville poultry semen extender; RFE-red fowl extender; TES-N-Tris-[hydroxymethyl]-methyl-2-aminoethansulfonic acid; LCM- Leningrad cryoprotective medium; BHSV-Blumberger Hahnen Spermna Verdünner); 2) type of packaging (P-pellets; S-straws); 3) cryoprotectant (Gly-glycerol; DMA-dimethylacetamide; DMF-dimethylformamide; MA-methylacetamide; SBL-soyabean lecithin; EY-egg yolk; DMSO-dimethylsulfoxide; EG-ethylenglycol; PND-propandiol; LDL-low-density lipoproteins); 4) concentration; 5) antioxidant (CLC-cyclodextrine; HA-hyaluronic acid; Glu-glutathion; Co-Q10-coenzyme Q10; P188-poloxamer 188; NLC-nanostructure lipid carriers); 6) freezing rate; 7) thawing temperature; 8) viability; 9) total motility; 10) fertility; 11)plasmtic membrane damage; 12) mitochondrial activity; 13) acrosomal integrity