Figure 2.
The C-terminal tail is dynamic but interacts with the NTD
(A) Top: order parameters (S2) for backbone amide N–H groups derived from 15N-relaxation data. An N–H group that is totally rigid in the molecular frame has an S2 value of 1, while a group with completely unrestricted motion has an S2 value of 0. Bottom: probabilities (P(ss)) of α-helical (upward-pointing red bars) and β-strand (downward-pointing green bars) secondary structure predicted by TALOS-N from the backbone chemical shifts.
(B and C) Long-range NOE contacts (black dashed lines) between the short N-terminal tail and the NTD.
(D–F) Long-range NOE contacts between the NTD and the long C-terminal tail. The dashed lines are labeled with the corresponding distances in the closest-to-the-mean structure.