REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
E.coli BL21(DE3)	Laboratory collection	N/A
E.coli Top10	Laboratory collection	N/A
Chemicals, peptides, and recombinant proteins
15NH4Cl	Merck (Sigma-Aldrich)	Cat # 299251
13C6-D-glucose	Merck (Sigma-Aldrich)	Cat # 389374
6xHis-TEV protease	Prepared in-house	N/A
DNAse	New England Biolabs	Cat #M0303S
Plasmid Mini Kit	Qiagen	Cat # 12123
Plasmid Mega Kit	Qiagen	Cat # 12181
T7 RNA polymerase	Laboratory collection	N/A
Deposited data
NMR Data of Nsp1	This Study	https://bmrb.io/ BMRB:34748
NMR Structure of Nsp1	This Study	https://www.wwpdb.org/ PDB: 8AOU
Oligonucleotides
PCR forward primer: ACTGAGAATCTTTATTTTCA G GGCGCCATGGAAAGCCTG GTT CCG GGTT	Microsynth	N/A
PCR reverse primer: GGTGGTGGTGGTGGTGC T CGA GTTAACCACCATTCA GTTCGCGCATCA	Microsynth	N/A
Recombinant DNA
pETM-11-Nsp1	This study	N/A
pHDV-5-SL1	Wacker et al.37	N/A
pHDV-5-SL1−4	Wacker et al.37	N/A
pSP64-5′UTR	Wacker et al.37	N/A
Software and algorithms
NMRPipe v.10.1	Delaglio et al.38	https://www.ibbr.umd.edu/nmrpipe/index.html
CcpNmr Analysis v2.4	Vranken et al.39	https://ccpn.ac.uk/software/analysisassign/
ARIA v2.3	Rieping et al.40	http://aria.pasteur.fr/
Pymol	Schrödinger	https://www.pymol.org/2/
Others
HisTrap HP column	GE Healthcare	GE17-5247-01
HiLoad Superdex 75 16/600column	GE Healthcare	GE28-9893-33
HiPrep Desalting column with Sephadex G-25 resin	Cytiva	Cytiva 17508702