TABLE 2.
Binding of recombinant Ib, truncated Ib variants, and Ia to Vero cells, as determined by fluorescence-activated cytometry
| Recombinant Ib or truncated variant | Binding to cell surface receptor (RFU)a | Docking of Ia to cell-bound Ib or truncated variant (RFU) |
|---|---|---|
| Ib | 17.6 | 23.1 |
| Ib 28-665 | 13.6 | NDb |
| Ib 107-665 | 14.6 | 8 |
| Ib 224-665 | 11.6 | 9.3 |
| Ib 358-665 | 12.1 | 11.1 |
| Ib 466-665 | 12.2 | 10.6 |
| Ib 558-665 | 7.6 | ND |
| Ib 1-66 | 6 | ND |
| Ib 1-323 | 7.2 | ND |
| Ib 1-466 | 8 | ND |
| Ib 1-631 | 7.2 | ND |
| Ib 1-655 | 5.2 | ND |
| None | 4.6 | 7.7 |
a
Geometric mean of the RFU. Results were considered positive if the RFU were at least twofold higher than the negative control (None) consisting of no Ib or truncated Ib variant but all other reagents.
b
ND, not determined.