TABLE 4.

Binding of Ib MAbs to truncated Ib variants by BIAcore

Protein	MAb binding (RU)a
	10A6	1D11	4H7	1E12	4F6	Control MAbb
Ib	342	639	285	234	299	87
Ib 107-665	0	664	−27	−29	532	10
Ib 224-665	−8	794	−37	−27	351	10
Ib 358-665	−3	563	−6	−2	632	5
Ib 466-665	−12	687	−34	−29	454	16
Ib 1-66	244	18	206	232	−43	36
Ib 1-323	242	−27	202	281	21	−13
Ib 1-466	274	−1	191	157	4	22
Ib 1-631	142	−8	117	121	5	3
Ib 1-655	181	144	133	147	131	7
HBS-EPc	−46	−36	−47	−36	22	6

^aMAbs captured by the RAM Fc surface varied between 1,130 and 1,730 RU. Values represent the RU due to MAb binding of the designated recombinant Ib or truncated variant. Results were considered positive if the RU were 2.5-fold or greater than that for the control MAb. 

^bControl MAb recognized C. botulinum neurotoxin A, but not Ib, as determined by an ELISA. 

^cHBS-EP, negative control containing only buffer and surfactant.