. 2001 Apr;69(4):2435–2441. doi: 10.1128/IAI.69.4.2435-2441.2001

TABLE 5.

Epitope mapping of MAbs against Ib by BIAcore

MAb 1^a	Binding of MAb 1 to RAM Fc	Binding of Ib to MAb 1	MAb 2^b
MAb 1^a	Binding of MAb 1 to RAM Fc	Binding of Ib to MAb 1	10A6	4H7	1E12	4F6	1D11
10A6	1,053	175	28	35	36	85	177
4H7	1,025	140	37	42	45	84	178
1E12	1,042	136	38	43	44	84	171
4F6	1,077	305	226	237	239	−35	469
1D11	1,255	395	217	227	217	420	15

MAb 1 (100 μg/ml) was added to a RAM Fc-coated sensor chip, followed by surface blocking, Ib (80 μg/ml), and MAb 2 (100 μg/ml). All values are presented in RU.

The RU after addition of MAb 2 indicate binding to the Ib-MAb 1 complex. MAb 1 and MAb 2 recognized the same epitope if binding of MAb 2 to Ib was ±10 RU of the value obtained when MAb 1 and MAb 2 were identical (boldface).