Figure 2.

Transcriptomic profiling of pituitary neuroendocrine tumors. (TOP) A transcriptome captures a snapshot in time of diverse transcripts present in a pituitary tumor cell. Microarrays capture a set of predetermined sequences whereas single-cell RNA sequencing and bulk RNA sequencing capture all transcripts using high-throughput sequencing. In contrast to bulk RNA sequencing, single-cell RNA sequencing can identify and analyze cell subpopulations. (BOTTOM) Transcriptomic analysis reveals differentially expressed genes that drive various tumor behaviors, such as hormone hypersecretion and tumor invasiveness. Transcriptomic changes in a corticotroph tumor cell are highlighted in this diagram.