FIG. 3.

Cytochrome c was not translocated from the mitochondria in cells actively or persistently infected with C. trachomatis (CT) after staurosporine challenge. HeLa cells actively and persistently infected with C. trachomatis serovar A/HAR-13 were treated with 1 μM staurosporine. The cells were sampled at 24-h intervals up to 120 h and fractionated into cytosolic and mitochondrial fractions. The actively infected HeLa cells represent the 48-h time point, while the persistently infected cells represent the 120-h time point. Western blots were probed with antibody specific for denatured cytochrome c and reacted with a secondary antibody conjugated with alkaline phosphatase. Arrow points to cytochrome c antibody.