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Flow chart of the proposed methodology (4-4 taxa classifier). FIT positive samples are subjected to microbiome profiling by 16S rRNA gene sequencing. Then a two phase classifier is applied. First the algorithm classifies CRC vs. non-CRC samples. Samples that are classified as non-CRC in the first phase are subjected to a second model that classifies CR vs. non-CR samples. FIT: fecal immunochemical test; CRC: colorectal cancer, CR: clinically relevant.
