Skip to main content
. 2022 Dec 23;13(1):52. doi: 10.3390/ani13010052

Figure 2.

Figure 2

(a) SDS-PAGE of multiple attempts to isolate lactoferrin (80 kDa) from seminal plasma (lanes 1–3). A second protein at 32 kDa consistently co-eluted with lactoferrin. Lane 4: Recombinant human lactoferrin. (b,c): Western Blots of SOD-3 LF. (b) Western blot using anti-SOD-3 antibody (1:1000, SCBT, sc-67088) identified the second protein as SOD-3. Lanes 1–3: Lactoferrin purified using different methods. Lane 4: Recombinant human lactoferrin. (c) Western Blot using anti-lactoferrin antibody (1:1000, SCBT, sc-14434) of purified lactoferrin (lane 1).