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Fluorescent (left panel) and visible light (right panel) of the same microscopic field (200× magnification). The images show that blocking with BSA for as little as 10 min before the addition of biot-BSA did not inhibit biot-LF/SOD-3 binding (upper panel) but resulted in the inhibition of biot-BSA binding to sperm (lower panel) indicating the specificity of LF to its receptors. The red circle identifies the same spermatozoa.
