TABLE 2.
Matching of primer sequences to known S. mutans templates
| Locus | RT primer (position) and sequence | PCR primer (position) and sequence | Coding sequencea | Length of PCR product (bp) |
|---|---|---|---|---|
| AR-186 | Ea1 (855) | Es8 (455) | ||
| 5′-TTTTATCCAGC | 5′-TGCCGATGAA | |||
| IIIIII | IIIII | 1∼2667 | 401 | |
| 5′-GAAAGTCCAGG | 5′-GCGTTATGAA | |||
| AR-731(icd)/AR-732(citZ) | Ea7 (2110) | Ea7 (1771) | ||
| 5′-TCTTTTTTACC | 5′-TCTTTTTTACC | 1798∼1916 (citZ) | ||
| IIIIIIIIII | IIIIIIII | 1918∼3099 (icd) | 340 | |
| U62799b | 5′-CTTTTTTTACC | 5′-CTATTTTTACC | ||
| GSP-781 | Ea7 (1244) | Es8 (896) | ||
| 5′-TCTTTTTTACC | 5′-TGCCGATGAA | |||
| I IIIII | I I III II | 1∼1295 | 349 | |
| 5′-CGGTAGTTACC | 5′-TTCTGATCAA |
a
The sequence of AR-186 was derived from the S. mutans genome sequence provided by the University of Oklahoma's Advanced Center for Genome Technology.
b
EMBL accession number.