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. 2022 Dec 22;12(1):39. doi: 10.3390/cells12010039

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Representative images of YFP-tagged wildtype OCT3 (top row), OCT3-D340G (middle row) and OCT3-R348W (bottom row) were captured by confocal microscopy to visualize the YFP-tagged transporter (left hand column), trypan blue (second column) and mTagBFP-tagged calnexin (CNX; third column). The overlay is shown in the right-hand column. (B) Western blot of lysates (6 µg) prepared from HEK293 cells expressing YFP-tagged wildtype OCT3, OCT3-D340G, OCT-R348W and from untransfected HEK293 cells. The red and the blue arrow indicate the position of the ER-resident core-glycosylated transporter and mature glycosylated transporter, respectively.