FIG. 4.

(A) Expression of a lacZ reporter located in synA in SA1 (diamonds) or SA2 (squares) or in ctrA in CA2 (triangles) during growth. Each time point shows the average value of three experimental measurements, and data are representative of three independent assays. (B) Expression of synA::lacZ reporters during mid-log growth phase (OD₅₅₀ ≈ 0.5) in different meningococcal backgrounds: 1, SA2, wild type; 2, SA2 in a ctrA::Ω background; 3, SA295, synA::lacZ with −95 to +339 promoter fragment of synA fused with lacZ-ermC and integrated into a noncoding chromosomal locus and retaining an intact capsule A and C region; 4, SA295 in a synA::Tn916 background; 5, lacZ-ermC cassette without promoter integrated into the same locus as SA295.