FIG. 5.

Transcriptional activities of synA::lacZ (SA2) and ctrA::lacZ (CA2) reporters in N. meningitidis containing mutations within 5′ UTR of synA. The mutations are shown in the diagram at the top and also noted in Fig. 1B. Each strain was grown in GC medium to mid-exponential phase, and β-galactosidase activities were assayed by the method of Miller (21). The values are presented as the percentage of the wild-type activity (SA2, 929.2 ± 158.0 U; CA2, 227.0 ± 29.8 U). Data shown are the averages of at least three independent experiments. Two-tailed unpaired Student's t test indicated that values for all mutants except SA293 and CA233 are statistically significantly different from that of the wild-type parent strain (P < 0.05).