TABLE 1.
Strains used in this study
| Strain | Relevant characteristicsa | Reference or source |
|---|---|---|
| E. coli | ||
| DH5α | Cloning host | 25 |
| CAB1 | K1 E. coli clinical isolate | Laboratory collection |
| N. meningitidis | ||
| NMB | B:2B:P1.2,5:L2 (CDC8201085), parent strain for all following constructs | 28 |
| 43 | synD::Tn916, class I insertion | 30 |
| M7 | synA::Tn916, class II insertion | 33 |
| 700 | ctrA::Tn916, class II insertion | 30 |
| ΩINT1 | Ω(Spr) cassette inserted into SnaBI site within intergenic region | This study |
| ΔΩINT1 | Same as ΩINT1 but with sequence between JS75 and JS74 primers deleted | This study |
| ΩINT2 | Ω(Spr) cassette inserted into EcoRI site 10 bp downstream of synA ATG start codon | This study |
| ΔΩINT2 | Same as ΩINT2 but with sequence between JS94 and JS93 primers deleted | This study |
| SA1 | lacZ-erm cassette inserted into SnaBI site within intergenic region | This study |
| SA2 | lacZ-erm cassette inserted into SnaBI site created within synA 339 bp from TTS | This study |
| CA2 | lacZ-ermC cassette inserted into SspI site within ctrA, 285 bp from TTS | This study |
| SA286 | SA2 strain with 70 bp between primers JS56 and JS86 deleted, removing both DR and IR | This study |
| CA286 | CA2 strain with same deletion as SA286 | This study |
| SA293 | SA2 strain with 46 bp between primers JS56 and JS93 deleted, removing only DR | This study |
| CA293 | CA2 strain with same deletion as SA293 | This study |
| SA233 | SA2 strain with 20 bp downstream of synA ATG start codon deleted, removing only IR | This study |
| CA233 | CA2 strain with same deletion as SA233 | This study |
| SA247 | SSA2 strain with 5 bp (TTATA) mutated to CCGGG within DR | This study |
| CA247 | CA2 strain with the same mutation as SA247 | This study |
| 988 | lacZ-ermC cassette inserted into chromosomal locus 120A1 | This study |
| SA295 | syn promoter fragment containing 95 bp upstream of TTS fused to lacZ-ermC cassette and integrated into 120A1 locus | This study |
a
TTS, transcriptional start site; DR, direct repeat; IR, inverted repeat.