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. 2022 Dec 23;12(1):82. doi: 10.3390/foods12010082

Table 1.

Conditions that facilitate the resuscitation process of VBNC cells.

Resuscitation Factors Bacterial Species Resuscitation Conditions References
VBNC-State Induction Condition Corresponding Resuscitation Condition
External stress removal Arcobacter butzleri, Aeromonas hydrophila, Staphylococcus aureus, Vibrio vulnificus, E. coli Low temperature Temperature up-shift [24,32,33,34,35,36]
Salmonella bovismorbificans, Enterococcus faecalis, Citrobacter sp., V. cholerae, Listeria monocytogenesisolates, Enterococci sp., Pasteurella piscicida, Yersinia pestis, V. shiloi, V. tasmaniensis, V. parahaemolyticus Starvation Addition of nutrients [29,37,38,39,40,41,42,43,44,45]
Enterobacter cloacae Desiccation Rewetting [46]
E. coli O157:H7, S. enterica serovar Typhimurium, L. monocytogenes Low pH Adjustment to the optimal pH [47]
Acetic acid bacteria, lactic acid bacteria O2 deprivation Addition of O2 [48]
E. coli O104:H4, Acidovorax citrulli, Erwinia amylovorain Copper Addition of chelating agent [49,50,51]
S. enterica, E. coli O157:H7 Food processing techniques Stress removal [26,52]
Supplementation with peroxidases Yeasts, Ralstonia solanacearum, E. coli O157:H7, Enterococcus sp., Salmonella sp. S. aureus, V. cincinnatiensis Catalase, sodium pyruvate, SOD, GST, CAT, acetaldehyde [53,54,55,56,57,58,59,60,61]
Host of VBNC cells Legionella pneumophila, E. coli O157:H7, Campylobacter jejuni, Helicobacter pylori, L. monocytogenes, V. cholerae O1, Francisella tularensis, E. faecalis, Campylobacter sp. Yolk sacs of embryonated eggs/1-week-old chicks, Caco-2 human intestinal epithelial cells, passage in the mouse intestine, co-culture with eukaryotic cells, injected intraperitoneally into mice, mice stomachs, co-culture with Acanthamoeba/Castellanii/Acanthamoeba polyphaga, ingestion by C. elegans, inoculated in iron-dextran-treated mice [9,11,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79]
Supplementation with substances that could promote resuscitation Salmonella typhimurium, E. coli O157:H7, Vibrio sp., V. parahaemolyticus Supplementation with autoinducer (AI) [6,80,81,82]
H. pylori, Mycobacterium tuberculosisare, Rhodococcus sp., actinobacteria, M. smegmatis, Sphingomonas and Pseudomonas, Rhodococcus biphenylivorans strain TG9T Supplementation with resuscitation promoting factor (Rpf) [83,84,85,86,87,88,89,90,91,92,93]