FIG. 3.

Growth and biofilm formation of S. parasanguis FW213 and its isogenic mutant strains. S. parasanguis strains FW213 (wild type), VT930 (fimA), VT1393 (fap1), VT1428 (fap1), VT1346 (pepO), and VT1354 (recA) were grown in TH to early stationary growth phase, washed once in sterile water, and then subcultured (1:200) into TH+Glu. Cultures were incubated in polystyrene microtiter dishes at 37°C, 5% CO₂ for 17 h. Crystal violet-stained wells are shown above the graph. Growth (light bars) and biofilm formation (dark bars) are quantified below as optical density at 490 and 562 nm, respectively. Assays were performed in quadruplicate; mean values and standard deviations are shown.