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. 2022 Dec 23;12(1):54. doi: 10.3390/cells12010054

Figure 2.

Figure 2

Composition and relative abundance of gut microbiota of mice subjected to chronic unpredictable mild stress (CUMS). The four groups are mice divided into no CUMS, CUMS (CUMS+ vehicle (0.9% saline)), CUMS mice treated with imipramine (CUMS + imipramine), and alkaloids (CUMS + alkaloids). (A) Chao1 index of imipramine and alkaloids-treated mice was reduced, but there was no significant difference in their diversity compared to saline treated CUMS mice. (B) The principal coordinates analysis (PCoA) based on the Bray–Curtis distance for GM showed a significant difference in microbial clusters among CUMS + imipramine and CUMS + alkaloids groups. Ellipses refer to 95% confidence intervals between treatments. (C) Taxonomic tree of GM represented in packed circles. The phylum level is denoted by the largest circles, while the inner circles are denoted by class, order, family, and genus. Firmicutes, Bacteroidetes, and Proteobacteria are found to be the dominant phyla. (D) Treatment with imipramine and alkaloids increased the Firmicutes, and decreased Bacteroidetes and Proteobacteria content. (E) Bar chart represents the specific changes in the relative abundance of GM between CUMS, CUMS + imipramine and CUMS + alkaloids groups at the genus level. * Denotes significant differences in specific microbes between groups. (F) Linear discriminant analysis scores on the cladograms of amplicon sequence variant is more than 2 based on linear discriminant analysis effect size [156].