Figure 9.

Myostatin regulates the molecular mechanism of myogenic differentiation by reducing DNA methylation. The upper right panel shows that knockdown of myostatin was achieved by constructing site-mutated vectors to transfect cells. The present work shows that deletion of myostatin triggers a decrease in global DNA methylation and promotes binding of the enzyme TET1 to the RACK1 promoter region, leading to hypomethylation and increased transcription in the RACK1 promoter region. Increased RACK1 promotes myogenic differentiation of bovine skeletal muscle satellite cells via activation of the PI3K/AKT/mTOR pathway.