Table 1.
Sequencing data by whole genome bisulfite sequencing (WGBS) for bovine MSTN+/−-edited (Z) and wild type (F).
| Groups | Sample | Clean Base (Gb) | Clean Reads | GC (%) | Q30 (%) | Mapped (%) | Bisulfite Conversion Rate (%) | Total_mC (%) |
|---|---|---|---|---|---|---|---|---|
| Wild type | F81199 | 155.23 | 1,037,577,664 | 22.77 | 90.56 | 76.30 | 98.66 | 0.29 |
| F81208 | 156.91 | 1,049,963,834 | 22.93 | 89.09 | 77.00 | 98.84 | 0.37 | |
| F81209 | 156.12 | 1,044,003,946 | 22.64 | 90.70 | 76.60 | 98.69 | 0.32 | |
| F81210 | 155.08 | 1,036,046,942 | 22.92 | 88.50 | 76.90 | 98.85 | 0.34 | |
| F81214 | 155.68 | 1,041,440,172 | 22.99 | 88.34 | 76.50 | 98.82 | 0.36 | |
| MSTN+/−-Edited | Z61004 | 156.31 | 1,046,166,382 | 23.17 | 87.40 | 75.68 | 99.20 | 0.36 |
| Z61020 | 155.89 | 1,042,125,536 | 23.24 | 90.11 | 74.25 | 98.76 | 0.37 | |
| Z61023 | 141.55 | 960,468,910 | 30.65 | 87.61 | 59.35 | 99.20 | 0.27 | |
| Z61117 | 151.37 | 1,016,262,024 | 23.29 | 88.51 | 75.24 | 99.20 | 0.34 | |
| Z61128 | 155.40 | 1,038,158,924 | 22.15 | 87.24 | 76.42 | 99.20 | 0.23 |
Global DNA methylation analysis of MSTN+/−-edited bovine (Z) and wild-type bovine (F) muscle using WGBS with 30× genome coverage and a bisulfite conversion efficiency of 98.66–99.20%. MSTN+/−-edited bovine and wild-type bovine muscles yielded an average of 152.11 and 155.80 Giga original bases, respectively. After filtering out low-quality data, each group yielded approximately 960 million clean reads, and the Q30 range of average reads per individual ranged from 87.24% to 90.70%. The mapped reads were used for subsequent analysis as the rates ranged from 59.35% to 76.90%. All methylated genomic C sites accounted for approximately 0.33% of the total bases in each group.