Skip to main content
. 2022 Dec 24;12(1):65. doi: 10.3390/cells12010065

Figure 2.

Figure 2

Pancreatitis is accompanied by a decrease in lncRNA-SNHG11. (A,B) The matrix (A) and volcano (B) maps of differential lncRNA obtained by RNA-seq in AP vs. sham. (C) The CTD database was used to screen pancreatitis-related genes and intersect with different lncRNAs to find the common gene SNHG11. (D–F) Expression of SNHG11 in the rats, AR42J and HPDE6-C7 assessed by qPCR (n = 3). (G,H) The stable overexpression strain of SNHG11 was constructed in AR42J and HPDE6-C7 cells, and the transfection effect was verified by qPCR experiment (n = 3). (I,J) The effect of SNHG11 on cell death induced by caerulein was observed by Trypan blue test in AR42J and HPDE6-C7(n = 3). (K,L) Cell proliferation was assessed by CCK8 assay in AR42J and HPDE6-C7 cell lines (n = 3). (M–R). Expression of IL-6, TNF-α, and IL-1β in the AR42J and HPDE6-C7 cell lines (empty vs. SNHG11) assessed by ELISA (n = 3). The data are presented as means ± SDs, * p < 0.05; ** p < 0.01; *** p < 0.001, between the indicated groups. ns = no significance.