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. 2022 Dec 24;12(1):65. doi: 10.3390/cells12010065

Figure 5.

Figure 5

MiR-7-5p binds to PLCB-1 and regulates its expression in pancreatitis. (A) The potential target mRNAs of miR-7-5p were predicted in a public database and intersected with RNA-seq data to find AP-related genes. (B–G) The expression of PLCB1 was explored in rat tissues, AR42J, and HPDE6-C7 cells by Q-PCR assay and WB assay. (H) Schematic diagram of the PLCB1-WT and PLCB1-Mut luciferase vectors. (I,J) Relative luciferase activities in AR42J and HPDE6-C7 cells co-transfected with PLCB1-WT or PLCB1-Mut and the miR-7-5p mimic, inhibitor, or corresponding negative control. (K,L) The interaction between miR-7-5p and PLCB1 was confirmed by RIP assay in AR42J and HPDE6-C7 cells. (M–R) The regulation effect of MiR-7-5p expression on PLCB1 was observed by qPCR assay and WB assay in AR42J and HPDE6-C7 cells. The data are presented as means ± SDs, n = 3 for each group, * p < 0.05; ** p < 0.01; *** p < 0.001, ns = no significance between the indicated groups.