Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Alexa Fluor® 700 anti-mouse CD45 (Clone 30-F11) | BioLegend | Cat# 103128; RRID: AB_493715 |
| PE/Cyanine7 anti-mouse CD31 (Clone 390) | BioLegend | Cat# 102418; RRID: AB_830757 |
| APC/Fire™ 750 anti-mouse CD326 (Clone G8.8) | BioLegend | Cat# 118229; RRID: AB_2629757 |
| Brilliant Violet 421™ anti-mouse CD140a (Clone APA5) | BioLegend | Cat# 135923; RRID: AB_2814036 |
| PE/Cyanine5 anti-mouse Ly-6A/E (Clone D7) | BioLegend | Cat# 108109; RRID: AB_313346 |
| Brilliant Violet 650™ anti-mouse/human CD11b (Clone M1/70) | BioLegend | Cat# 101259; RRID: AB_2566568 |
| PerCP/Cyanine5.5 anti-mouse CD3ε (Clone 145–2C11) | BioLegend | Cat# 100328; RRID: AB_893318 |
| PE/Cyanine5 anti-mouse CD4 (Clone RM4–5) | BioLegend | Cat# 100514; RRID: AB_312717 |
| Brilliant Violet 650™ anti-mouse CD8a (Clone 53–6.7) | BioLegend | Cat# 100742; RRID: AB_2563056 |
| Alexa Fluor® 647 anti-mouse NK-1.1 (Clone PK136) | BioLegend | Cat# 108720; RRID: AB_2132713 |
| Brilliant Violet 711™ anti-mouse IFN-γ (Clone: XMG1.2) | BioLegend | Cat# 505836; RRID: AB_2650928 |
| PE/Cyanine7 anti-human/mouse Granzyme B (Clone QA16A02) | BioLegend | Cat# 372214; RRID: AB_2728381 |
| PE anti-mouse Perforin (Clone S16009A) | BioLegend | Cat# 154306; RRID: AB_2721639 |
| PE/Cyanine7 anti-mouse Ki-67 (Clone: 16A8) | BioLegend | Cat# 652425; RRID: AB_2632693 |
| CD9 (Clone: KMC8) | Thermo Fisher Scientific | Cat# 14–0091-82; RRID: AB_823138 |
| CD63 (Clone: 446703) | R&D Systems | Cat# MAB5417-SP; RRID: AB_2076610 |
| CD81 (Clone: 431301) | R&D Systems | Cat# MAB4865-SP; RRID: AB_2275889 |
| α-Smooth Muscle-FITC (Clone: 1A4) | Sigma-Aldrich | Cat# F3777; RRID: AB_476977 |
| Cleaved Caspase-3 (Asp175) | Cell Signaling Technology | Cat# 9661S; RRID: AB_2341188 |
| PE anti-mouse/human CD11b (Clone: M1/70) | BioLegend | Cat# 101207; RRID: AB_312790 |
| Alexa Fluor® 647 anti-mouse Ly-6G (Clone: 1A8) | BioLegend | Cat# 127610; RRID: AB_1134159 |
| CCL2 (MCP-1) | Thermo Fisher Scientific | Cat# PA5–115555; RRID: AB_2893318 |
| Phospho-S6 (Ser235/236) (Rabbit) | Cell Signaling Technology | Cat# 5316S; RRID: AB_10694989 |
| CD82 | Thermo Fisher Scientific | Cat# PA5–13228; RRID: AB_2244577 |
| HIF-1 alpha (Clone: ESEE122) | Thermo Fisher Scientific | Cat# 14–9100-82; RRID: AB_11217480 |
| HILPDA | Biorbyt | Cat# orb389656 |
| GAPDH | Cell Signaling Technology | Cat# 5174S; RRID: AB_10622025 |
| CD161c/NK1.1 | Thermo Fisher Scientific | Cat# BS-4682R; RRID: AB_11092712 |
| PE anti-mouse CD45 (Clone QA17A26) | BioLegend | Cat# 157603; RRID: AB_2876536 |
| eFluor™ 660 anti-mouse CD11b (Clone M1/70) | Thermo Fisher Scientific | Cat# 50–0112-82; RRID: AB_11218507 |
| Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP | Thermo Fisher Scientific | Cat# 31460; RRID: AB_228341 |
| Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP | Thermo Fisher Scientific | Cat# 31430; RRID: AB_228307 |
| Goat anti-Rat IgG (H+L) Secondary Antibody, HRP | Thermo Fisher Scientific | Cat# 31470; RRID: AB_228356 |
| Goat anti Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 594 | Thermo Fisher Scientific | Cat# A11037; RRID: AB_2534095 |
| Anti-mouse NK1.1 (Clone: PK136) | Bio X Cell | Cat# BE0036; RRID: AB_1107737 |
| Mouse IgG2a isotype control (Clone: C1.18.4) | Bio X Cell | Cat# BE0085; RRID: AB_1107771 |
| Polyclonal Armenian hamster IgG | Bio X Cell | Cat# BE0091; RRID: AB_1107773 |
| Anti-mouse IL-1β (Clone B122) | Bio X Cell | Cat# BE0246; RRID: AB_2687727 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Collagenase, Type IV, powder | Worthington Biochemical | Cat# LS004188 |
| Dnase I | MilliporeSigma | Cat# DN25 |
| LIVE/DEAD™ Fixable Aqua Dead Cell Stain | Thermo Fisher Scientific | Cat# L34966 |
| BODIPY 493/503 | Thermo Fisher Scientific | Cat# D3922 |
| BODIPY FL C16 | Thermo Fisher Scientific | Cat# D3821 |
| LipidTOX Red | Thermo Fisher Scientific | Cat# H34476 |
| LysoTracker™ Red DND-99 | Thermo Fisher Scientific | Cat # L7528 |
| Recombinant Murine IL-1β | PeproTech | Cat# 211–11B |
| Human IL-1β | Invivogen | Cat# rcyec-hil1b |
| Recombinant Human IL-1RA | BioLegend | Cat# 553902 |
| Recombinant Murine IL-12 p70 | PeproTech | Cat# 210–12 |
| Recombinant Murine IL-15 | PeproTech | Cat# 210–15 |
| Recombinant Mouse IL-18 | BioLegend | Cat# 767002 |
| Recombinant Murine CCL2 | PeproTech | Cat# 250–10 |
| D-Luciferin Firefly, potassium salt | Gold Biotechnology | Cat# LUCK |
| Doxorubicin hydrochloride | MilliporeSigma | Cat# PHR1789 |
| Cisplatin | MilliporeSigma | Cat# PHR1624 |
| Paclitaxel | MilliporeSigma | Cat# PHR1803 |
| Prostaglandin E2 | Cayman Chemical | Cat# 14010 |
| Etomoxir | Cayman Chemical | Cat# 11969 |
| IOX4 | Cayman Chemical | Cat# 18181 |
| PX-478 | Cayman Chemical | Cat# 10005189 |
| Atglistatin | Cayman Chemical | Cat# 15284 |
| BAY 59–9435 | MedChem Express | Cat# HY-102056 |
| JZL184 | Cayman Chemical | Cat# 13158 |
| SN50 Peptide | Cayman Chemical | Cat# 17493 |
| MLN120B | Cayman Chemical | Cat# 32819 |
| BAY 11–7082 | Cayman Chemical | Cat# 10010266 |
| Cycloheximide | Cayman Chemical | Cat# 14126 |
| LY294002 | Cayman Chemical | Cat# 70920 |
| Genistein | Millipore Sigma | Cat# G6649 |
| Chlorpromazine | Cayman Chemical | Cat# 16129 |
| Critical commercial assays | ||
| Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate | R&D Systems | Cat# MLB00C |
| Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit | R&D Systems | Cat# MJE00B |
| Proteome Profiler Mouse XL Cytokine Array | R&D Systems | Cat# ARY028 |
| Prostaglandin E2 Parameter Assay Kit | R&D Systems | Cat# KGE004B |
| Triglyceride Colorimetric Assay Kit | Cayman Chemical | Cat# 10010303 |
| Lipase Activity Assay Kit | Cayman Chemical | Cat# 700640 |
| Free Fatty Acid Fluorometric Assay Kit | Cayman Chemical | Cat# 700310 |
| Glucose Colorimetric Detection Kit | Thermo Fisher Scientific | Cat# EIAGLUC |
| L-Amino Acid Quantitation Kit | Millipore Sigma | Cat# MAK002–1KT |
| Diacylglycerol Assay Kit | Abcam | Cat# ab242293 |
| Phosphatidylcholine Assay Kit | Abcam | Cat# ab83377 |
| Phosphatidylethanolamine Assay Kit | Abcam | Cat# ab241005 |
| Phosphatidylglycerol Assay Kit | Abcam | Cat# ab273288 |
| Phosphatidylinositol Assay Kit | Abcam | Cat# ab252900 |
| Sphingomyelin Quantification Colorimetric Assay Kit | Abcam | Cat# ab287856 |
| Lipid Hydroperoxide (LPO) Assay Kit | Cayman Chemical | Cat# 705002 |
| PKH26 Red Fluorescent Cell Linker Mini Kit | MilliporeSigma | Cat# MINI26–1KT |
| Invitrogen™ Total Exosome Isolation Reagent | Thermo Fisher Scientific | Cat# 44–783-59 |
| Direct-zol RNA Miniprep Plus w/ TRI Reagent | Zymo | Cat# R2073 |
| High-Capacity cDNA Reverse Transcription Kit | Thermo Fisher Scientific | Cat# 4368814 |
| PowerUp SYBR™ Green PCR Master Mix | Thermo Fisher Scientific | Cat# A25778 |
| Deposited data | ||
| RNA-seq data of ex vivo cultured lung MCs | Reference 37 | ArrayExpress: E-MTAB-11887 |
| scRNA-seq data of lung CD140a+ MCs | This paper | ArrayExpress: E-MTAB-11902 |
| RNA-seq data of lung metastases and matched primary tumors | Reference 45 | GEO: GSE110590 |
| RNA-seq data of lung metastases and other organ metastases | Reference 46 | GEO: GSE14018 |
| Data S1 – Source Data | This study | N/A |
| Experimental models: Cell lines | ||
| 4T1 | ATCC | Cat# CRL2539 |
| AT3 | Gift from S.I. Abrams | N/A |
| E0771 | CH3 Biosystems | Cat# 940001 |
| YAC-1 | ATCC | Cat# TIB-160 |
| NK-92MI | ATCC | Cat# CRL2408 |
| K562 | ATCC | Cat# CCL243 |
| MDA-4175 | Gift from Y. Kang | N/A |
| 4T1-Luc | Reference 10 | N/A |
| AT3-Luc | Reference 10 | N/A |
| E0771-Luc | Reference 10 | N/A |
| MDA-4175-Luc | This paper | N/A |
| AT3-mCherry | Reference 10 | N/A |
| Human lung mesenchymal cells | Sciencell | Cat# 7540 |
| Experimental models: Organisms/strains | ||
| C57BL/6J | The Jackson Laboratory | JAX: 000664 |
| BALB/cJ | The Jackson Laboratory | JAX: 000651 |
| NSG: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ | The Jackson Laboratory | JAX: 005557 |
| CD140aEGFP: B6.129S4-Pdgfratm11(EGFP)Sor/J | The Jackson Laboratory | JAX: 007669 |
| Atglflox/flox (B6N.129S-Pnpla2tm1Eek/J) | The Jackson Laboratory | JAX: 024278 |
| Hilpdaflox/flox (Hilpdatm1.1Nat/J) | The Jackson Laboratory | JAX: 017360 |
| Hif1aflox/flox (B6.129-Hif1atm3Rsjo/J) | The Jackson Laboratory | JAX: 007561 |
| PdgfraCre: C57BL/6-Tg(Pdgfra-cre)1Clc/J | The Jackson Laboratory | JAX: 013148 |
| PdgfraCre; Atglflox/flox | This paper | N/A |
| PdgfraCre; Hilpdaflox/flox | This paper | N/A |
| PdgfraCre; Hif1aflox/flox | This paper | N/A |
| PdgfaCre; Atglflox/flox; MMTV | This paper | N/A |
| MMTV-PyMT: B6.FVB-Tg(MMTV-PyVT)634Mul/LellJ | The Jackson Laboratory | JAX: 022974 |
| FVB/N-Tg(MMTV-PyVT)634Mul/J | The Jackson Laboratory | JAX: 002374 |
| Il1r1−/−: B6.129S7-Il1r1tm1Imx/J | The Jackson Laboratory | JAX: 003245 |
| Oligonucleotides | ||
| See Table S1 for primer sequences | ||
| Recombinant DNA | ||
| pLenti CMV Puro LUC (w168–1) | Reference 93 | Addgene# 17477 |
| pLV-mCherry | Tsoulfas lab | Addgene# 36084 |
| Software and algorithms | ||
| BD FACSDiva software | BD Biosciences | https://www.bdbiosciences.com/ |
| FlowJo (version 10.8.0) | BD Biosciences | https://flowjo.com |
| R (version 4.0.2) | The R Foundation | https://www.r-project.org/ |
| Seurat (version 3.2.2) | Satija Lab | https://satijalab.org/seurat/ |
| Metascape | Reference 96 | https://metascape.org/ |
| LungMAP | LungMAP | https://www.lungmap.net/ |
| GraphPad Prism (version 8.2.1) | GraphPad Software | https://www.graphpad.com/ |
| Biorender | Biorender | https://biorender.com/ |
| ImageJ (1.53c) | ImageJ | https://imagej.nih.gov/ij/ |
| Other | ||