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Complementation of GT20 cytopathogenicity defect to macrophages by htrA mutant constructs, with monolayer viability measured by Alamar Blue dye reduction following infection at an MOI of 0.5. (a) Complementation of GT20 (T20) by mutant constructs harboring point mutations within the catalytic domain. (b) Complementation of GT20 (T20) by PDZ deletion mutant constructs. These data are representative of at least three independent experiments.
