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. 2001 Apr;69(4):2569–2579. doi: 10.1128/IAI.69.4.2569-2579.2001

FIG. 6.

FIG. 6

FIG. 6

Rescue of intracellular replication of the dotA mutant (GL10) but not the htrA mutant (GT20) by the parental strain AA100. Colocalization of (A) AA100 and GT20(gfp) and (B) AA100 and GL10(gfp) within communal phagosomes, determined by scanning laser confocal microscopy. Red, anti-L. pneumophila antiserum (α-Lpn); green, gfp-GT20 or gfp-GL10 (gfp). Arrows indicate GT20 or GL10 (a dotA mutant) which cannot be rescued within a communal phagosome coinhabited by AA100. (c and d) Infected phagosomes were scored as follows. AA100, AA100 alone within a phagosome; GT20 or GL10, mutants alone within a phagosome; AA100+GT20 or AA100+GL10, AA100 and mutants colocalized within the same phagosome; AA100+/GT20− or AA100+/GL10−, colocalization of AA100 and mutants within the same phagosome, with proficient replication of AA100 but no replication of the mutant; Rescue, intracellular replication of mutants within a communal phagosome with AA100. Phagosomes were scored for all macrophages in randomly selected fields. These data are representative of three independent experiments performed in triplicate.