FIG. 5.

IFN-γ secreted by granuloma cells or spleen cells after in vitro culture in the presence of α-CD3 antibody. (A) Granuloma cells or (B) spleen cells isolated after 6 weeks of BCG infection by the indicated strains were cultured in vitro for 3 days in the presence of α-CD3 (145-2C11) (5 μg/ml) to activate TCR-mediated signaling. IFN-γ secreted into culture supernatants was measured by ELISA. Values shown are the averages of quadruplicate measurements from one experiment, and error bars represent the standard error of the mean. Statistical significance was analyzed by student's t test. ∗, P < 0.05. Results shown are representative of three independent experiments.