Skip to main content
. 2001 Apr;69(4):2604–2611. doi: 10.1128/IAI.69.4.2604-2611.2001

FIG. 4.

FIG. 4

Quenching of TAMRA fluorescent bacteria by trypan blue. N. gonorrhoeae N303 isolates were stained by incubating them 30 min with TAMRA in the dark. After washing, the fluorescence of the labeled bacteria was determined by flow cytometry (hatched) and the mean fluorescence of the labeled bacteria was calculated at 1,271. Trypan blue was then added to the bacteria and the fluorescence was again measured (black line). The mean fluorescence after addition of the dye was calculated at 45. The reduction in the mean fluorescence corresponds to a quenching of 97% of the initial fluorescence. This is a representative experiment of at least three different experiments with similar results.

HHS Vulnerability Disclosure