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. 2001 Apr;69(4):2604–2611. doi: 10.1128/IAI.69.4.2604-2611.2001

TABLE 1.

FACS analysis of H. pylori phagocytosis inhibitiona

Strain(s) Phagocytosis (%)b
N303 76 ± 8
N303 + cytochalasin D 37 ± 14
P12 + N303 35 ± 11
PAI + N303 69 ± 4
a

JOSKM cells were incubated with TAMRA-labeled N303 with or without cytochalasin D. Alternatively, JOSKM cells were infected with H. pylori P12 or PAI followed by an infection with TAMRA-labeled N303. The mean fluorescence of the JOSKM-infected cells was determined before and after the addition of trypan blue, which inhibited the fluorescence of extracellular attached bacteria. 

b

The percentage of fluorescence reduction after addition of the dye corresponds to the phagocytosis of N303. Results are the means ± the standard deviations of at least three different experiments. 

HHS Vulnerability Disclosure