TABLE 1.
FACS analysis of H. pylori phagocytosis inhibitiona
Strain(s) | Phagocytosis (%)b |
---|---|
N303 | 76 ± 8 |
N303 + cytochalasin D | 37 ± 14 |
P12 + N303 | 35 ± 11 |
PAI + N303 | 69 ± 4 |
JOSKM cells were incubated with TAMRA-labeled N303 with or without cytochalasin D. Alternatively, JOSKM cells were infected with H. pylori P12 or PAI followed by an infection with TAMRA-labeled N303. The mean fluorescence of the JOSKM-infected cells was determined before and after the addition of trypan blue, which inhibited the fluorescence of extracellular attached bacteria.
The percentage of fluorescence reduction after addition of the dye corresponds to the phagocytosis of N303. Results are the means ± the standard deviations of at least three different experiments.