TABLE 1.
Effects of BSO on E. coli-induced inflammatory responses in the mouse livera
| Antigen | Treatment | Positive cellsb
|
||
|---|---|---|---|---|
| KC (cells/HPF) | Hep (cells/HPF) | EC (intensity score) | ||
| TNF-α | Vehicle | 17.5 ± 1.9 | — | 0 |
| BSO | 0.6 ± 0.8∗ | — | 0 | |
| iNOS | Vehicle | 16.7 ± 2.0 | 41.8 ± 3.9 | 1 |
| BSO | 15.0 ± 1.9 | 38.3 ± 5.3 | 1 | |
| ICAM-1 | Vehicle | 23.0 ± 3.0 | — | 3 |
| BSO | 19.7 ± 3.1 | — | 3 | |
Female C3HeB/FeJ mice (six per group) were challenged i.p. with 4 × 107 CFU of E. coli O111:B4 bacteria, and their livers were recovered either 1.5 h (for TNF-α staining) or 6 h (for iNOS and ICAM-1 staining) later. Mice were treated with either 0.15 M NaCl (vehicle) or 5 mmol of BSO/kg at 6 h and again at 3 h prior to challenge with bacteria. At least 5 randomly selected HPF (×400; HPF = 0.11 mm2) were scored for each tissue section.
Values are means ± SD. KC, Kupffer cell; Hep, hepatocyte; EC, sinusoidal endothelial cell. Semiquantitative scoring of endothelial cell staining used an intensity range from 0 to 3 (see Materials and Methods). ∗, this group differed significantly from the vehicle control group (P < 0.01). —, none detected.