TABLE 3.
Role of IFN-γ in regulating E. coli-induced hepatic inflammatory mediator expression in DEM-treated micea
| Treatmentb | Mouse strain | Serum IFN-γ (pg/ml) | Antigen (cell type)-positive cells/HPFc
|
Intensity score
|
|||
|---|---|---|---|---|---|---|---|
| iNOS (Hep) | iNOS (KC) | ICAM-1 (KC) | iNOS (EC) | ICAM-1 (EC) | |||
| Vehicle | IFN-γ+/+ | 3,360 | 38.4 ± 9.9 | 20.3 ± 3.2 | 20.0 ± 3.5 | 3 | 3 |
| DEM | IFN-γ+/+ | 275 | 33.6 ± 9.8 | 18.7 ± 5.5 | 23.3 ± 7.4 | 3 | 3 |
| Vehicle | IFN-γ−/− | <33 | 4.1 ± 6.2∗ | 16.4 ± 1.0 | 1.0 ± 1.1∗ | 1 | 1 |
| DEM | IFN-γ−/− | <33 | 1.7 ± 1.4∗ | 13.4 ± 3.4 | 1.9 ± 0.5∗ | 1 | 1 |
a
BALB/c wild-type or mutant IFN-γ-null mice (five per group) were treated with vehicle or DEM and challenged with E. coli 2 h later. Liver tissue and blood samples were recovered 6 h later. Shown are the average values from two experiments. The detection limit of the IFN-γ assay was 33 pg/ml. ∗, this group differed significantly from the corresponding wild-type group (P < 0.01). Also see Table 1, footnote a.
b
See Fig. 1.
c
Values are means ± SD.