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No induction of pIL8LUC by alpha-toxin in p65/p50 knockout cells. 3T3 wild-type cells (black columns) or 3T3 cells carrying a p65/p50 double-knockout phenotype (white columns) were transiently transfected with pIL8LUC and were treated with media alone, alpha-toxin (40 μg/ml), or PMA. Luciferase activity in cell lysates was measured. The results are from three independent experiments; error bars indicate standard deviation.
