TABLE 1.
Animal group and time postchallenge (h) | Semiquantitative score for cell type:
|
||||
---|---|---|---|---|---|
γδ+ | CD4+ | CD8+ | MHC-II | B | |
Immune | |||||
0.5 | + | + | ++ | + | + |
1 | ++ | + | +++ | + | + |
2 | ND | + | ++ | ++ | + |
4 | − | + | ++ | ++ | + |
8 | ND | ++ | +++++ | ++++ | ++ |
24 | − | +++ | +++++ | +++ | +++ |
Nonimmune | |||||
0.5 | − | + | + | + | + |
1 | − | + | + | + | + |
2 | ND | + | + | + | + |
4 | − | + | + | + | + |
8 | ND | + | + | + | + |
24 | − | + | + | + | + |
Analysis of the cell type present was performed by immunoperoxidase staining using monoclonal antibodies. Values represent a semiquantitative score as expressed by the cell number per field magnified ×40 in infected tissues and are scored as follows: −, no cells seen on multiple sections; +, 1 to 5 cells present; ++, 6 to 10 cells present; +++, 11 to 20 cells present; ++++, 20 to 50 cells present; +++++, >50 cells present. ND, not done.