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. 2022 Dec 28;24(1):486. doi: 10.3390/ijms24010486

Figure 2.

Figure 2

JQ1 reduced renal type IV collagen overexpression at the gene and protein levels in nephrotoxic nephritis. Mice were treated daily with JQ1 (100 mg/kg/day) or vehicle, starting before the first NTS injection. (A) RNA was isolated from frozen whole kidney samples, and α3 chain type IV collagen (Col4a3); type I collagen (ColI); fibronectin, and Ccn2 gene expression levels were evaluated by real-time qPCR. (B) Protein levels of COLIV were quantified by Western blot in total kidney extracts. The figure shows representative blots where GAPDH was used as a loading control. Data are expressed as the mean ± SEM of 5–7 animals per group. * p < 0.05 vs. control; # p < 0.05 vs. NTS-injected mice. (C) Immunohistochemistry with an anti-type IV collagen antibody in paraffin-embedded kidney sections. A representative animal from each group was shown (200× magnification). A detailed image of glomeruli was included (600× magnification). (D) Type IV collagen staining quantification. Scale bar represents 100 μm (200×) and 50 μm (600×).