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. 2022 Dec 28;24(1):486. doi: 10.3390/ijms24010486

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibition of BET proteins by JQ1 prevents kidney SOX9 and ECM overexpression induced by UUO. (A,B) Gene and protein expression levels of SOX9 were evaluated by real-time qPCR (A) and Western blot (B). (C) The nuclear localization of SOX9 and its relationship with the extracellular matrix was evaluated by immunofluorescence. D, E) Inhibition of BET proteins by JQ1 decreases profibrotic markers in murine UUO such as (D) Fibronectin, Pai-1, and Tgf-β1, evaluated by real-time qPCR, or (E) The protein levels of Fibronectin and Type I Collagen (COLI) determined by Western Blot. Data are expressed as the mean ± SEM of 6–8 animals per group and scale bar represents 20 μM. * p < 0.05 vs. contralateral; # p < 0.05 vs. obstructed kidneys treated with JQ1.