Table A1.

Cytotoxicity effect of analyzed flavonoid series on selected cancer cell lines in in vitro assays.

Cell Line	CHRY			API			KAE			QUE			MIR
	IC50	Assay	Ref	IC50	Assay	Ref	IC50	Assay	Ref	IC50	Assay	Ref	IC50	Assay	Ref
MDA-MB-468	5.84 ± 0.67 μM	MTT, 72 h	[235]	8.9 μg/mL	72 h	[237]	23 μg/mL	72 h MTT	[238]	18.1 + 1.6 μM	TIA	[239]
MDA-MB-231							47.0 + 2.9 μM	TIA	[239]	>100 μM	MTT, 24 h	[240]	114.75 µM	MTT, 72 h	[241]
	40 (35–45) μM	TBE + CTG 72 h	[242]	75.12 ± 1.85 μM	MTT. 24 h	[243]	34 μg/mL	MTT, T72 h	[238]	>200 μM	MTT, 24 h	[243]	30.29 ± 1.87 μM	MTT, 24 h	[243]
							>200	MTT, 24 h	[243]	101 (82–124) μM	TBE + CTG 72 h	[242]	>200 μM	TBE + CTG 72 h	[242]
							38 μM	TBE + CTG 72 h	[242]
MCF-7	4.20 ± 1.70 μM	MTT, 72 h	[235]	37.2 ± 3.7 μM	WST-1, 48 h	[244]	25 (16–40) μM	TBE + CTG 72 h	[242]	37 μM	MTT, 24 h	[240]	>200 μM	TBE + CTG 72 h	[242]
	34 (28–40) μM	TBE + CTG 72 h	[242]	7.8 μg/mL	72 h	[237]				102 (62–161) μM	TBE + CTG 72 h	[242]
	25.6 ± 1.2 µM	MTT, 72 h	[245]							>80 µM	CQ, 72 h	[246]
										13.7 ± 0.6 µM	MTT, 72 h	[245]
HeLa	86.51 ± 2.9	MTT, 48 h	[247]	10 µM	MTT, 48 h	[248]	50 µM	MTT, 48 h	[249]	35.5 ± 1.1 µM	MTT, 72 h	[250]	9.06 µM	MTT, 24 h	[251]
				41.0 ± 4.5	WST-1.48 h	[244]
				74.71 ± 3.6 µg/mL	MTT, 48 h.	[252]
HepG2	10.69 ± 0.94 μM	MTT, 72 h	[235]	57.86 ± 2.9 µg/mL	MTT, 48 h	[252]	30.92 μM	RFR, 24 h	[253]	87 μM	TBE, 18 h	[254]	87.84 μM	CCK-8, 72 h	[255]
				37.4 ± 3.8	WST-1, 48 h	[244]
HT-29	126.5 ± 6.3	SG, 72 h	[256]	130.6 ± 6.8	SG, 72 h	[256]	136.9 ± 7.4	SG, 72 h	[256]	85.59 ± 8.6 μM	SG, 72 h	[256]	47.6 ± 2.3 µM	SG, 72 h	[256]
Caco-2	4.84 0.19 µM	MTT, 72 h	[235]	115.4 ± 10.3 μM	SG 72 h	[256]	163.2 ± 1.6 μM	SG, 72 h	[256]	96.3 ± 6.7 μM	SG 72 h	[256]	88.4 ± 3.4 µM.	SG, 72 h	[256]
	115.6 ± 2.1 μM	SG, 72 h	[256]
HCT-116	4.07 ± 0.29 µM	MTT, 72 h	[235]	34.4 ± 3.6 µM	WST-1, 48 h	[244]	7.2 μM	WST-8, 72 h	[257]	40 µM	MTT, 48 h	[258]	No effect	MTT, 72 h	[241]
LNCaP							75 µM	MTT, 48 h	[258]	25 µM	DC, 72 h	[259]	53.8 μM	xC, 24 h	[260]
	56.81 µM	CQ, 72 h	[246]				28:8 ± 1:5 μM	MTT, 48 h	[261]	19.44 µM	CQ, 72 h	[246]
				20 µM	DC, 72 h	[259]	33.3 μM	xC, 24 h	[260]
PC-3				50 µM	DC, 72 h	[259]	52.24 µM	CQ 72 h	[246]	14.5 μM	xC 24 h	[260]	47.6 μM	CCK-8, 48 h	[262]
				>20 μM	MTT, 48 h	[263]	58:3 ± 3:5 μM	MTT, 48 h	[261]	50 µM	DC, 72 h	[259]
	>100 µM	CQ, 72 h	[246]	38.8 ± 3.9	WST-1, 48 h	[244]	41.98 µM	CQ, 72 h	[246]	33.41 µM	CQ, 72 h	[246]
A549	4.64 ± 1.36 µM	MTT, 72 h	[235]	93.7 ± 3.7 μM	MTT, 4 h	[264]	72 μM	MTT, 24 h	[265]	8.65 µg/mL	24 h, MTT	[266]	154.3 ± 4.6	CCK-8, 48 h	[267]
	49.2 ± 0.6 µM	WST-8, 48 h	[268]	35.4 ± 3.7	WST-1, 48 h	[244]

(MTT) 2,5-diphenyl-2H-tetrazolium bromide assay (xC) xCELLigence system, (TBE) trypan blue exclusion, (CQ) CyQuant, (CTG) Cell Titer Glo (Promega), (RFR) reduction resazurin to fluorescent resorufin, (TIA) [3H]-thymidine incorporation assay, (DB) DC Bio-Rad assay (total cellular protein concentration), (SG) SYTOX-Green, (WST-8) (CCK-8) water-soluble tetrazolium salt assay.