Figure 4.

IPF treatments impair invadosome formation and function. IPF fibroblasts were incubated in the presence of nintedanib (n = 7). (A) Representative images of fibroblasts from the same patient plated on fluorescent gelatin (green) and stained for nuclei (blue) and f-actin (red). Scale bar = 50 µm. (B) The percentage of cell-forming invadosomes and (C) the surface degraded by fibroblasts (n = 4) were calculated. (D) Cortactin and f-actin clusters were counted (n = 4). (E) The percentage of invadosome-forming fibroblasts was determined in the presence of pirfenidone (n = 4). * p < 0.05, ** p < 0.01, **** p < 0.0001.