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. 2022 Dec 29;24(1):582. doi: 10.3390/ijms24010582

Figure 8.

Figure 8

Effect of β-End 1–31 C-terminal derivatives, enkephalins and classical σ1R ligands on σ1R-σ2R and σ1R-NR1 C1 associations. Upper panel: amino acid sequences of physiological β-End C-terminal derivatives. The effects of increasing concentrations of (A) β-End C-terminal 27–31, 28–31, 30–31, and (B) classical σ1R ligands, such as the antagonists S1RA, BD1047, and progesterone, and the agonists PRE084, PPCC, and pregnenolone sulfate, were studied on σ1R-σ2R and σ1R-NR1 C1 associations. Data were compared to that of the control, which was obtained in the absence of ligands. Lower panel: amino acid sequences of β-End N-terminal derivative 1–5 [methionine-enkephalin (Met-Enk)] and its aminopeptidase product 2–5. The sequences of 1–5 leucine-enkephalin (Leu-Enk) and 2–5 metabolite are also indicated. (C,D) Effects of Met-Enk, Leu-Enk, their chimeric αN-acetylated forms and 2–5 metabolites on σ1R-σ2R and σ1R-NR1 C1 associations. Data were compared to that of the control, which was obtained in absence of ligands. (A–D) For details, see Section 4 and Figure 7. Data are the mean ± SD, n = 3.