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. 2022 Dec 29;24(1):596. doi: 10.3390/ijms24010596

Figure 1.

Figure 1

Effect of atropine and 4-DAMP on proliferation, apoptosis, and production of choline in CT-26 cells. CT-26 cells were treated with different concentrations of atropine at different time points (A). Number of viable cells after 8 h incubation with various concentrations of atropine and 4-DAMP (B). CT-26 cells were treated with various concentrations of carbachol and donepezil for 8 h (C). The amount of choline was measured in CT-26 cells treated with atropine, 4-DAMP, and donepezil (D). Annexin V-FITC/PI staining of CT-26 murine colon cancer cell line treated with control (E), atropine (E’), and 4-DAMP (E”). Values in (A–E) are mean ± standard error of the mean (SEM) from at least three independent experiments performed in triplicate wells. Two independent experiments were performed in triplicates. Values presented as mean ± SEM from at least two independent experiments for choline assay. Two-way ANOVA followed by Tukey’s multiple comparison test was used and considered significant when * p < 0.05.