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. 2022 Dec 29;24(1):606. doi: 10.3390/ijms24010606

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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DDR in normal human tissue organoids after BaP treatment. Organoids from gastric D95 and D88; (A), pancreatic D39 and D44; (B), kidney D50 and D21; (C), colon D351 and D311; (D) and liver (D4 undifferentiated and differentiated; (E) tissues were treated with the indicated BaP concentrations for 48 h, and lysates were analysed by Western blotting. Various DDR proteins (p-p53, pCHK2, p21 and γ-H2AX) were detected and GAPDH was used as a loading control. cBoB + Cis (cBoB treated with 3.125 μM cisplatin) was used as a positive control. Representative blots are shown (n = 2).