TABLE 3.
Internalization of subclones of pMMB33Inv by HeLa cells
Strain | Insert length (kb) | Insert genes | No. of intracellular bacteria/5 × 105 cellsa |
---|---|---|---|
E. coli C600 | <1 | ||
E. coli C600(pMMB33Inv) | 20 | Entire csg cluster | 1.58 × 105 |
E. coli C600(pMRInv) | 8.5 | Entire csg cluster | 3.43 × 105 |
E. coli C600(pMSa) | 6 | csgBAC csg DEF | <1 |
E. coli C600(PMRBg) | 10.5 | csgBAC csgDEF (G disrupted) | <1 |
The experiment was performed as described in Materials and Methods.