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. 2022 Dec 28;24(1):480. doi: 10.3390/ijms24010480

Figure 5.

Figure 5

Identification of Arabidopsis overexpressing the ZmDHN15 gene. (A) pCAMBIA3301- ZmDHN15 vector map. (B) Untransformed plant and transgenic plants. (C) Test strip detection. Col-0: Untransformed plant, 1–8: turn pCAMBIA3301-ZmDHN15 positive plants. (D) PCR detection of bar gene in transgenic Arabidopsis thaliana, M: DNA Marker DL 2000, P: pCAMBIA3301-ZmDHN15 recombinant plasmid, N: Negative control, Col-0: Untransformed plant, 1–8: turn pCAMBIA3301-ZmDHN15 positive plants. (E) qRT-PCR validation of transgenic Arabidopsis thaliana. Data were expressed as the mean of triplicate values, and the error represented the SD. Asterisks indicate statistically significant differences: p < 0.05 (*) and p < 0.01 (**).